DDDDK-Tag(binds to flag sequnence) rabbit pAb
簡(jiǎn)要描述:DDDDK-Tag(binds to flag sequnence) rabbit pAb宿主: Polyclonal, Rabbit,IgG反應(yīng)性: Species independent應(yīng)用: WB;ELISA;IP;IF
產(chǎn)品型號(hào): YP-Ab-17192
所屬分類:標(biāo)簽內(nèi)參抗體
更新時(shí)間:2024-10-31
詳細(xì)說(shuō)明:
DDDDK-Tag(binds to flag sequnence) rabbit pAb
抗體,也叫免疫球蛋白(Ig),是一種能特異性結(jié)合抗原的糖蛋白,而抗原是在易感染動(dòng)物體內(nèi)引發(fā)抗體產(chǎn)生的物質(zhì)。在體內(nèi),抗體是由于外源性分子的侵襲而產(chǎn)生的。抗體以一個(gè)或者多個(gè)Y字形單體存在,每個(gè)Y字形單體由4條多肽鏈組成,包含兩條相同的重鏈和兩條相同的輕鏈。輕鏈和重鏈?zhǔn)歉鶕?jù)它們的分子量大小來(lái)命名的。Y字形結(jié)構(gòu)的頂端是可變區(qū),為抗原結(jié)合部位。任何一個(gè)抗體的輕鏈都可以分為κ或λ型(基于小分子多肽結(jié)構(gòu)上的差異),每一個(gè)抗體的重鏈則決定了它的類或型。
產(chǎn)品詳情
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
免疫原詳細(xì)信息
DDDDK synthetic peptide conjugated to KLH.
相關(guān)試劑
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
背景
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
天津俊達(dá)生物科技有限公司,是從事實(shí)驗(yàn)室耗材銷售的現(xiàn)代化生物科技企業(yè),公司本著“誠(chéng)信為本,互利共贏"的核心價(jià)值觀,秉承“以市場(chǎng)為導(dǎo)向,提供服務(wù)"的宗旨為各大院校、科研單位、第三方實(shí)驗(yàn)室檢測(cè)機(jī)構(gòu)等提供的產(chǎn)品和服務(wù)。目前公司主要經(jīng)營(yíng)分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等生物學(xué)產(chǎn)品,多種實(shí)驗(yàn)室耗材 歡迎垂詢!
備注:圖片僅供參考。僅供科研,不可用于臨床。